Rat Fas L ELISA Kit

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Rat Fas L ELISA Kit
Rat Fas L ELISA Kit standard Curve

Rat Fas L ELISA Kit

Catalog No : 3030062


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SKU: 3030062. Categories: , , .

Rat Fas L ELISA Kit Features

Product Name            Rat Fas L ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   40 pg/ml
Assay Range               82-60000 pg/ml
Reactivity                     Rat
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Faslg, Apt1Lg1, CD95-L, Fasl, Tnfsf6, Tnlg1a
Gene ID                        25385
Swissprot ID              P36940

Recovery

Sample Type    Average % RecoveryRange %
Serum113106-132
Plasma10899-125
Cell culture media9281-101

Linearity

Sample TypeDilution% of Expected
Serum1:2105
1:498
1:893
Plasma1:2110
1:4107
1:897
Cell culture media1:2112
1:4111
1:8102

Standard Curve: Rat Fas L ELISA Kit

Rat Fas L ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintenance of immune privileged sites. Tumor cells can evade immune surveillance by up-regulating Fas Ligand to kill tumor infiltrating lymphocytes. In mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.

Related Products

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Rat Fas L ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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