Catalog No : 3030056
Product Name Rat EGF ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum, Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity <1 pg/ml
Assay Range 0.82-200 pg/ml
Reactivity Rat
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names Egf
Gene ID 25313
Swissprot ID P07522
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 90 | 79-106 |
Plasma | 99 | 89-112 |
Cell culture media | 97 | 83-108 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 80 |
1:4 | 81 | |
1:8 | 85 | |
Plasma | 1:2 | 79 |
1:4 | 80 | |
1:8 | 87 | |
Cell culture media | 1:2 | 92 |
1:4 | 87 | |
1:8 | 83 |
Standard Curve: Rat EGF ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Rat EGF ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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