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Rat CXCL2 ELISA Kit
Rat CXCL2 ELISA Kit Standard Curve

Rat CXCL2 ELISA Kit

Catalog No : 3030344


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SKU: 3030344. Categories: , .

Rat CXCL2 ELISA Kit Features

Product Name            Rat CXCL2 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   2 pg/mL
Assay Range                8.2-2000 pg/mL
Reactivity                     Rat
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names:              Cxcl2, Mip-2; Scyb2
Gene ID                         114105
Swissprot ID               P30348

Recovery

Sample Type    Average % RecoveryRange %
Serum8881-97
Plasma9285-103
Cell culture media8478-96

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:480
1:886
Plasma1:293
1:479
1:884
Cell culture media1:296
1:482
1:892

Standard Curve: Rat CXCL2 ELISA Kit

Rat CXCL2 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils. CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Rat CXCL2 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Rat

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