Rat CINC-1 ELISA Kit

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Rat CINC-1 ELISA Kit
Rat CINC-1 ELISA Kit

Rat CINC-1 ELISA Kit

Catalog No : 3030100


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SKU: 3030100. Categories: , .

Rat CINC-1 ELISA Kit Features

Product Name            Rat CINC-1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   220 pg/ml
Assay Range               234.4-15000 pg/ml
Reactivity                     Rat
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Cxcl1, CINC-1,  Gro1
Gene ID                         81503
Swissprot ID               P14095

Recovery

Sample Type    Average % RecoveryRange %
Serum9987-108
Plasma10595-121
Cell culture media11498-122

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:4123
1:8117
Plasma1:299
1:489
1:892
Cell culture media1:284
1:478
1:882

Standard Curve: Rat CINC-1 ELISA Kit

Rat CINC-1 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines.  CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis.CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, arteriogenesis, inflammation, wound healing, and tumorigenesis. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2. An initial study in mice showed evidence that CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

Related Products

Mouse KC ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Rat CINC-1 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Rat

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