Product Name Rat Agrin ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum , Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity 50 pg/mL
Assay Range 0.069-50 ng/ml
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names Agrn, ARG
Gene ID 25592
Swissprot ID P25304
|Sample Type||Average % Recovery||Range %|
|cell culture media||79||70-87|
|Sample Type||Dilution||% of Expected|
|cell culture media||1:2||88|
Standard Curve: Rat Agrin ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. This protein has nine domains homologous to protease inhibitors. It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.
Agrin binds to several receptors on the surface of skeletal muscle. The receptor that seems to be required for formation of the neuromuscular junction (NMJ) is called the MuSK receptor. MuSK is a receptor tyrosine kinase. A protein called rapsyn is then recruited to the primary MuSK scaffold, to induce the additional clustering of acetylcholine receptors (AChR). This is thought of as the secondary scaffold. A protein called Dok-7 has shown to be additionally required for the formation of the secondary scaffold; it is apparently recruited after MuSK phosphorylation and before acetylcholine receptors are clustered.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Rat Agrin ELISA Kit is for Research Use Only.
1 Plate Kit, 2 Plate Kit, 5 Plate Kit