Rat Agrin ELISA Kit

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Rat Agrin ELISA Kit

Rat Agrin ELISA Kit

Catalog No : 3030343


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Rat Agrin ELISA Kit Features

Product Name           Rat Agrin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum , Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   50 pg/mL
Assay Range                0.069-50 ng/ml
Reactivity                     Rat
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Agrn, ARG
Gene ID                         25592
Swissprot ID               P25304

Recovery

Sample Type    Average % RecoveryRange %
Serum   8776-98
Plasma   8978-101
cell culture media   7970-87

Linearity

Sample TypeDilution% of Expected
Serum1:2110
1:4115
1:8107
Plasma1:2110
1:4102
1:8103
cell culture media1:288
1:485
1:890

Standard Curve: Rat Agrin ELISA Kit

Rat Agrin ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. This protein has nine domains homologous to protease inhibitors. It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.
Agrin binds to several receptors on the surface of skeletal muscle. The receptor that seems to be required for formation of the neuromuscular junction (NMJ) is called the MuSK receptor. MuSK is a receptor tyrosine kinase. A protein called rapsyn is then recruited to the primary MuSK scaffold, to induce the additional clustering of acetylcholine receptors (AChR). This is thought of as the secondary scaffold. A protein called Dok-7 has shown to be additionally required for the formation of the secondary scaffold; it is apparently recruited after MuSK phosphorylation and before acetylcholine receptors are clustered.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Rat Agrin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Rat

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