Rat Adiponectin ELISA Kit

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Rat Adiponectin ELISA Kit
Rat Adiponectin ELISA Kit

Rat Adiponectin ELISA Kit

Catalog No : 3030001


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Rat Adiponectin ELISA Kit Features

Product Name           Rat Adiponectin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   0.6 ng/mL
Assay Range                0.82-200 ng/ml
Reactivity                     Rat
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ADIPOQ; ACDC; ACRP30;
Gene ID                         246253
Swissprot ID               Q8K3R4

Recovery

Sample Type    Average % RecoveryRange %
Serum   11298-132
cell culture media   116102-132

Linearity

Sample TypeDilution% of Expected
Serum1:2105
1:4114
1:8113
cell culture media1:2112
1:4117
1:8109

Standard Curve: Rat Adiponectin ELISA Kit

Rat Adiponectin ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Adiponectin, also called Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers.

Related Products

Human Adiponectin EasyTest™ ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Rat Adiponectin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Rat

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