Porcine IFN-alpha ELISA Kit

Porcine IL-12 ELISA Kit
Porcine IL-12 ELISA Kit
July 9, 2017
Equine IL-2 ELISA Kit
July 9, 2017
Show all
Porcine IFN-alpha ELISA Kit
Porcine IFN-alpha ELISA Kit

Porcine IFN-alpha ELISA Kit

Catalog No : 3070476


Clear
SKU: 3070476. Categories: , .

Porcine IFN-alpha ELISA Kit Features

Product Name            Porcine IFN-alpha ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   20 pg/mL
Assay Range               20.5-5000 pg/mL
Reactivity                     Porcine
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               IFN-ALPHA-14, IFN-alpha
Gene ID                        100310804
Swissprot ID              K7GNH9

Recovery

Sample Type    Average % RecoveryRange %
Serum 8677-101
Plasma 8980-97
Cell culture media 9481-109

Linearity

Sample TypeDilution% of Expected
Serum1:2116
1:4125
1:8126
Plasma1:2118
1:4132
1:8124
Cell culture media1:2105
1:4102
1:8112

Standard Curve: Porcine IFN-alpha ELISA Kit

Porcine IFN-alpha ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Interferon-alpha, also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Porcine IFN-alpha ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Porcine (pig)

Reviews

There are no reviews yet.

Be the first to review “Porcine IFN-alpha ELISA Kit”