Porcine CCL4 ELISA Kit

Mouse TWEAK R ELISA Kit
Mouse TWEAK R ELISA Kit
September 8, 2016
Porcine IL-13 ELISA Kit
Porcine IL-13 ELISA Kit
September 8, 2016
Show all
Porcine CCL4 ELISA Kit
Porcine CCL4 ELISA Kit Standard Curve

Porcine CCL4 ELISA Kit

Catalog No : 3070369


Clear
SKU: 3070369. Categories: , , .

Porcine CCL4 ELISA Kit Features

Product Name            Porcine CCL4 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   0.2 ng/mL
Assay Range                0.205-50 ng/mL
Reactivity                     Porcine
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names              CCL4, MIP-1BETA, MIP1b, SCY4A
Gene ID                         396668
Swissprot ID             Q711P4

Recovery

Sample Type    Average % RecoveryRange %
Serum10596-124
Plasma10294-118
cell culture media111101-126

Linearity

Sample TypeDilution% of Expected
Serum1:2101
1:4103
1:898
Plasma1:2107
1:4108
1:8102
Cell culture media1:2111
1:497
1:895

Standard Curve: Porcine CCL4 ELISA Kit

Porcine CCL4 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor. CCL4 has been shown to interact with CCL3.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Porcine CCL4 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Porcine (pig)

Reviews

There are no reviews yet.

Be the first to review “Porcine CCL4 ELISA Kit”