Mouse Prolactin EasyTest™ ELISA Kit

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Mouse Prolactin EasyTest™ ELISA Kit
Mouse Prolactin ELISA Kit Standard Curve

Mouse Prolactin EasyTest™ ELISA Kit

Catalog No : 1020020


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SKU: 1020020. Category: .

Product Features

Product Name           Mouse Prolactin EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              2.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   80pg/mL
Assay Range                0.82-200ng/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               AV290867, Gha11a1, Prl
Gene ID                        19109
Swissprot ID               P06879

 

Recovery

Sample Type    Average % RecoveryRange %
Serum  8776-102
Plasma   9592-98

Linearity

Sample TypeDilution% of Expected
Serum1:293
1:4115
1:886
Plasma1:299
1:495
1:898

Standard Curve: Mouse Prolactin EasyTest™ ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim mouse Prolactin mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family. Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. It is associated with human milk production.

Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. It has important cell cycle-related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor, binding to cytokine-like receptors, it influences hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Prolactin EasyTest™ ELISA Kit.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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