Product Name Mouse Leptin EasyTest™ ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Process One wash step, two incubations
Assay Length 2.5 hours
Sample Volume Serum , Plasma, 50µL
Detection Method Colorimetric
Assay Range 65.5-16000pg/mL
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names Lep; Ob; obese
Gene ID 16846
Swissprot ID P41160
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Standard Curve: Mouse Leptin EasyTest™ ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Bioaim mouse Leptin mix
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of Bioaim mix.
4. Incubate 2 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.
Leptin receptor activation inhibits neuropeptide Y (NPY) and agouti-related peptide (AgRP), and activates α-melanocyte-stimulating hormone (α-MSH). The NPY neurons are a key element in the regulation of hunger; small doses of NPY injected into the brains of experimental animals stimulates feeding, while selective destruction of the NPY neurons in mice causes them to become anorexic. Conversely, α-MSH is an important mediator of satiety, and differences in the gene for the α-MSH receptor are linked to obesity in humans. Leptin interacts with six types of receptors (Ob-Ra–Ob-Rf, or LepRa-LepRf), which in turn are encoded by a single gene, LEPR.Ob-Rb is the only receptor isoform that can signal intracellularly via the Jak-Stat and MAPK signal transduction pathways, and is present in hypothalamic nuclei. Generally, leptin is thought to enter the brain at the choroid plexus, where the intense expression of a form of leptin receptor molecule could act as a transport mechanism.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Mouse Leptin EasyTest™ ELISA Kit is for Research Use Only.
1 Plate Kit, 2 Plate Kit, 5 Plate Kit