Mouse IgG2b ELISA Kit

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Mouse IgG2b ELISA kit
Mouse IgG2b ELISA kit

Mouse IgG2b ELISA Kit

Catalog No : 3020484


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SKU: 3020484. Category: .

Mouse IgG2b ELISA Kit Features

Product Name            Mouse IgG2b ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   10 pg/mL
Assay Range                12.3-3000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules

Recovery

Sample Type    Average % RecoveryRange %
Serum11795-126
Plasma9482-107
Cell culture media8073-91

Linearity

Sample TypeDilution% of Expected
Serum1:280
1:479
1:882
Plasma1:286
1:488
1:883
Cell culture media1:291
1:487
1:882

Standard Curve: Mouse IgG2b ELISA Kit

Mouse IgG2b ELISA kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Antibodies are major components of humoral immunity. IgG is the main type of antibody found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.

Immunoglobulin G-antibody responses to bacterial capsular polysaccharide antigens can be almost completely restricted to IgG2, and IgG2 deficiency may result in the virtual absence of IgG anti-carbohydrate antibodies, although these responses can also be compensated for by enhanced levels of other IgG subclasses, in particularly by elevated IgG1 and IgG3 levels. An increased susceptibility to certain bacterial infections is associated with IgG2 deficiency, suggesting a role of IgG2 in the defense to these pathogens. Low concentrations of IgG2 often occur in association with a deficiency in IgG4 and/or IgA1 and IgA2.

In a mouse model of autoantibody mediated anemia using IgG isotype switch variants of an anti erythrocytes autoantibody, it was found that mouse IgG2a was superior to IgG1 in activating complement. Moreover, it was found that the IgG2a isotype was able to interact very efficiently with FcgammaR. As a result, 20 times higher doses of IgG1, in relationship to IgG2a autoantibodies, were required to induce autoantibody mediated pathology.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse IgG2a ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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