Mouse IGF-1 ELISA Kit

Mouse G-CSF ELISA Kit
Mouse G-CSF ELISA Kit
April 11, 2016
Mouse 4-1BB ELISA Kit
Mouse 4-1BB ELISA Kit
April 11, 2016
Show all
Mouse IGF-1 ELISA Kit
Mouse IGF-1 ELISA Kit Standard Curve

Mouse IGF-1 ELISA Kit

Catalog No : 3020025


Clear

Mouse IGF-1 ELISA Kit Features

Product Name            Mouse IGF-1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   <1 pg/ml
Assay Range               2.7-2000 pg/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               C730016P09Rik, Igf-1, Igf-I
Gene ID                         16000
Swissprot ID              P05017

Recovery

Sample Type    Average % RecoveryRange %
Serum9995-102
Plasma110105-124
Cell culture media8779-96

Linearity

Sample TypeDilution% of Expected
Serum1:2120
1:4117
1:8102
Plasma1:2121
1:4113
1:8112
Cell culture media1:2117
1:4115
1:8107

Standard Curve: Mouse IGF-1 ELISA Kit

Mouse IGF-1 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to at least two cell surface receptors: the IGF-1 receptor (IGF1R), and the insulin receptor. Insulin-like growth factor 1 receptor (IGF-1R) and other tyrosine kinase growth factor receptors signal through multiple pathways. A key pathway is regulated by phosphatidylinositol-3 kinase (PI3K) and its downstream partner, the mammalian target of rapamycin (mTOR).

Related Products

Human IGF-I ELISA Kit

Rat IGF-1 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse IGF-1 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Reviews

There are no reviews yet.

Be the first to review “Mouse IGF-1 ELISA Kit”