Mouse IGF-1 EasyTest™ ELISA Kit

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Mouse IGF-1 EasyTest™ ELISA Kit
Mouse IGF-1 ELISA Kit Standard Curve

Mouse IGF-1 EasyTest™ ELISA Kit

Catalog No : 1020025


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Product Features

Product Name            Mouse IGF-1 EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   10pg/mL
Assay Range                32.8-8000pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               C730016P09Rik; Igf-1; Igf-I
Gene ID                         16000
Swissprot ID              P05017

 

Recovery 

Sample Type    Average % RecoveryRange %
Serum 9988-113
Plasma   106103-113

Linearity

Sample TypeDilution% of Expected
Serum1:2104
1:493
1:8113
Plasma1:2106
1:485
1:8112

Standard Curve: Mouse IGF-1 EasyTest™ ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim mouse IGF-1 mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to at least two cell surface receptors: the IGF-1 receptor (IGF1R), and the insulin receptor. Insulin-like growth factor 1 receptor (IGF-1R) and other tyrosine kinase growth factor receptors signal through multiple pathways.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Mouse IGF-1 EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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