Mouse gp130 ELISA Kit

Mouse GITR L ELISA Kit
Mouse GITR L ELISA Kit
September 8, 2016
Mouse IgE ELISA Kit
Mouse IgE ELISA Kit
September 8, 2016
Show all
Mouse gp130 ELISA Kit
Mouse gp130 ELISA Kit Standard Curve

Mouse gp130 ELISA Kit

Catalog No : 3020322


Clear

Mouse gp130 ELISA Kit Features

Product Name            Mouse gp130 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   30 pg/mL
Assay Range                41-10000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Il6st, 5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699e, gp130
Gene ID                         16195
Swissprot ID               Q00560

Recovery

Sample Type    Average % RecoveryRange %
Serum 9989-110
Plasma9791-104
Cell culture media11094-121

Linearity

Sample TypeDilution% of Expected
Serum1:2113
1:4116
1:8109
Plasma1:2102
1:4110
1:8107
Cell culture media1:287
1:498
1:8101

Standard Curve: Mouse gp130 ELISA Kit

Mouse gp130 ELISA kit standard curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the classI cytokine receptor family. Binding of IL-6 or IL-11 to either the membrane-anchored or soluble IL-6 R or IL-11 R, respectively, initiates the association of IL-6 R (IL-11 R) with gp130. The complex then undergoes homodimerization and signal transduction. For other IL-6 family cytokines, such as LIF and OSM, signal transduction is similarly triggered by the heterodimerization of gp130 with LIFR or OSM R. gp130 has no intrinsic tyrosine kinase activity. Instead, it is phosphorylated on tyrosine residues after complexing with other proteins. The phosphorylation leads to association with JAK/Tyk tyrosine kinases and STAT protein transcription factors. In particular, STAT-3 is activated which leads to the activation of many downstream genes. Other pathways activated include RAS and MAPK signaling.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse gp130 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

Reviews

There are no reviews yet.

Be the first to review “Mouse gp130 ELISA Kit”