Mouse Galectin-7 ELISA Kit

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Mouse Galectin-7 ELISA Kit
Mouse Galectin-7 ELISA Kit standard Curve

Mouse Galectin-7 ELISA Kit

Catalog No : 3020200


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Mouse Galectin-7 ELISA Kit Features

Product Name            Mouse Galectin-7 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   30 pg/mL
Assay Range                65.5-16000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Lgals7, Galectin-7, Pig1
Gene ID                        16858
Swissprot ID              O54974

Recovery

Sample Type    Average % RecoveryRange %
Serum 125109-137
Plasma10792-118
Cell culture media128110-132

Linearity

Sample TypeDilution% of Expected
Serum1:287
1:488
1:890
Plasma1:292
1:493
1:8110
Cell culture media1:2101
1:498
1:8102

Standard Curve: Mouse Galectin-7 ELISA Kit

Mouse Galectin-7 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. It is expressed at all stages of epidermal differentiation. The protein was found mainly in stratified squamous epithelium. The antigen localized to basal keratinocytes, although it was also found, albeit at lower levels, in the suprabasal layers where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell–cell and/or cell–matrix interactions necessary for normal growth control.

Related Products

Human Galectin-7 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Galectin-7 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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