Mouse Epiregulin ELISA Kit

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Mouse Epiregulin ELISA Kit
Mouse Epiregulin ELISA Kit Standard Curve

Mouse Epiregulin ELISA Kit

Catalog No : 3020318


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Mouse Epiregulin ELISA Kit Features

Product Name            Mouse Epiregulin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   6 pg/mL
Assay Range               8.2-6000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Ereg, EPR
Gene ID                        13874
Swissprot ID              Q61521

Recovery

Sample Type    Average % RecoveryRange %
Serum 7875-87
Plasma8177-92
Cell culture media10191-113

Linearity

Sample TypeDilution% of Expected
Serum1:2118
1:4120
1:8121
Plasma1:2116
1:4117
1:8101
Cell culture media1:287
1:485
1:889

Standard Curve: Mouse Epiregulin ELISA Kit

Mouse Epiregulin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), Transforming Growth Factor (TGF)-alpha, Amphiregulin (AR), HB (heparin-binding)-EGF, Betacellulin (BTC), and the various heregulins (HRGs). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB family receptors (ErbB1 through B4). Epiregulin is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Epiregulin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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