Mouse Endoglin ELISA Kit

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Mouse Endoglin ELISA Kit
Mouse endoglin ELISA Kit

Mouse Endoglin ELISA Kit

Catalog No : 3020184


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Mouse Endoglin ELISA Kit Features

Product Name            Mouse Endoglin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   15 pg/mL
Assay Range                16.4-4000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ENG, AI528660, AI662476, CD105, Endo, S-endoglin
Gene ID                         13805
Swissprot ID               Q63961

Recovery

Sample Type    Average % RecoveryRange %
Serum   118107-126
Plasma   11496-127
cell culture media  9888-110

Linearity

Sample TypeDilution% of Expected
Serum1:2117
1:4125
1:8112
Plasma1:2108
1:4122
1:8115
cell culture media1:2107
1:4117
1:8110

Standard Curve: Mouse Endoglin ELISA Kit

Mouse endoglin ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida family of proteins. Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane and cytoplasmic domains. It is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3, but only after binding T beta RII.

Similarly, they interact with Activin A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-IA/ALK-3 or BMPR-IB/ALK-6. BMP-9, however, is reported to bind endoglin directly. Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP 7 signals in the same myoblast cell line. In endothelial cells, endoglin inhibits T beta RI/ALK 5, but enhances ALK 1-mediated activation. In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (Flt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Endoglin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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