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Mouse EGFR ELISA Kit

Mouse EGF R ELISA Kit

Catalog No : 3020057


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Mouse EGF R ELISA Kit Features

Product Name           Mouse EGF R ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   0.2 ng/ml
Assay Range               0.205-50 ng/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               EGFR; 9030024J15Rik, AI552599, Erbb, Errb1, Errp, Wa5, wa-2, wa2
Gene ID                        13649
Swissprot ID              Q01279

Recovery

Sample Type    Average % RecoveryRange %
Serum 10895-127
Plasma 10592-116
Cell culture media 11192-124

Linearity

Sample TypeDilution% of Expected
Serum1:2113
1:4114
1:8112
Plasma1:2107
1:4122
1:8117
Cell culture media1:2110
1:4127
1:8115

Standard Curve: Mouse EGF R ELISA Kit

Mouse EGFR ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

The EGF R subfamily of receptor tyrosine kinases comprises four members: EGF R , ErbB2 , ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins. An extracellular ligand binding domain contains two cysteine-rich domains and a cytoplasmic domain. The cytoplasmic domain contains a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2; which results in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis.

Related Products

Mouse EGF ELISA Kit

Human EGF ELISA Kit

Human EGF R ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Mouse EGF R ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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