Mouse E-Cadherin ELISA Kit

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Mouse E-Cadherin ELISA Kit
Mouse E-Cadherin ELISA Kit Standard Curve

Mouse E-Cadherin ELISA Kit

Catalog No : 3020316


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Mouse E-Cadhein ELISA Kit Features

Product Name            Mouse E-Cadhein ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   4 pg/mL
Assay Range                4.1-3000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               cdh1, AA960649, ARC-1, E-cad, Ecad, L-CAM, UVO, Um
Gene ID                         12550
Swissprot ID               P09803

Recovery

Sample Type    Average % RecoveryRange %
Serum 8075-89
Plasma9081-101
Cell culture media112102-124

Linearity

Sample TypeDilution% of Expected
Serum1:280
1:4101
1:896
Plasma1:281
1:4112
1:8101
Cell culture media1:285
1:4107
1:8105

Standard Curve: Mouse E-Cadherin ELISA Kit

Mouse E-Cadherin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Cadherin-1 also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. In epithelial cells, E-cadherin-containing cell-to-cell junctions are often adjacent to actin-containing filaments of the cytoskeleton. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid, and ovarian cancers.

Related Products

Custom human E-Cadherin ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse E-Cadherin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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