Mouse Decorin ELISA Kit

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Mouse Decorin ELISA Kit
Mouse Decorin ELISA Kit Standard Curve

Mouse Decorin ELISA Kit

Catalog No : 3020053


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Mouse Decorin ELISA Kit Features

Product Name            Mouse Decorin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   4 pg/mL
Assay Range                4.1-1000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Dcn, DC, DSPG2, PG40, PGII, PGS2, SLRR1B
Gene ID                         13179
Swissprot ID               P28654

Recovery

Sample Type    Average % RecoveryRange %
Serum 10597-121
Plasma9484-107
Cell culture media10889-129

Linearity

Sample TypeDilution% of Expected
Serum1:2103
1:4101
1:8110
Plasma1:2104
1:4108
1:898
Cell culture media1:2101
1:4110
1:895

Standard Curve: Mouse Decorin ELISA Kit

Mouse Decorin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Decorin is a proteoglycan that is on average 90 – 140 kDa. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression.

Related Products

Human Decorin ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Decorin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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