Mouse CXCL15 ELISA Kit

Mouse Chordin ELISA Kit
Mouse Chordin ELISA Kit
October 28, 2017
Mouse Endostatin ELISA Kit
October 29, 2017
Show all
Mouse CXCL15 ELISA Kit
Mouse CXCL15 ELISA Kit

Mouse CXCL15 ELISA Kit

Catalog No : 3020354


Clear
SKU: 3020354. Categories: , .

Mouse CXCL15 ELISA Kit Features

Product Name            Mouse CXCL15 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   0.8 ng/mL
Assay Range                0.82-200 ng/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Cxcl15, Il8; weche; Scyb15; lungkine
Gene ID                         20309
Swissprot ID               Q9WVL7

Recovery

Sample Type    Average % RecoveryRange %
Serum120109-132
Plasma123116-133
Cell culture media125117-136

Linearity

Sample TypeDilution% of Expected
Serum1:2123
1:4115
1:8112
Plasma1:2123
1:4121
1:8117
Cell culture media1:2118
1:492
1:8101

Standard Curve: Mouse CXCL15 ELISA Kit

Mouse CXCL15 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Mouse Lungkine/CXCL15, also named WECHE, is a member of the ELR motif-containing CXC chemokines. The gene for mouse Lungkine has been mapped to chromosome 5. By Northern blot and in situ hybridization, Lungkine transcripts are only specifically detected in the adult and fetal lung, and its expression is up regulated under inflammatory condition. Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking. Studies from Lungkine knockout mice suggest that Lungkine is an important mediator of neutrophil migration from the lung parenchyma into the airspace. Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Chordin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

Reviews

There are no reviews yet.

Be the first to review “Mouse CXCL15 ELISA Kit”