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Mouse Chordin ELISA Kit
Mouse Chordin ELISA Kit

Mouse Chordin ELISA Kit

Catalog No : 3020313


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Mouse Chordin ELISA Kit Features

Product Name            Mouse Chordin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   45 pg/mL
Assay Range                49.2-12000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Chrd, Chd
Gene ID                         12667
Swissprot ID               Q9Z0E2

Recovery

Sample Type    Average % RecoveryRange %
Serum7771-83
Plasma7969-87
Cell culture media9682-110

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:495
1:893
Plasma1:289
1:4101
1:892
Cell culture media1:2101
1:4104
1:8111

Standard Curve: Mouse Chordin ELISA Kit

Mouse Chordin ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Chordin is a bone morphogenetic protein antagonist composed of four small cysteine-rich domains, whose function is not known. It is considered as a key developmental protein that dorsalizes early vertebrate embryonic tissues and it dorsalizes the developing embryo by binding ventralizing TGF-β proteins such as bone morphogenetic proteins. It may also play a role in organogenesis. There are five named isoforms of this protein that are produced by alternative splicing.

In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Chordin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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