Mouse CD30L ELISA Kit

Mouse CD30 ELISA Kit
Mouse CD30 ELISA Kit
September 7, 2016
Mouse CD40 ELISA Kit
Mouse CD40 ELISA Kit
September 7, 2016
Show all
Mouse CD30L ELISA Kit
Mouse CD30L ELISA Kit Standard Curve

Mouse CD30L ELISA Kit

Catalog No : 3020312

SKU: 3020312. Categories: , .

Mouse CD30L ELISA Kit Features

Product Name            Mouse CD30L ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   < 1 pg/mL
Assay Range                0.41-100 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names              Tnfsf8, CD153, CD30LG, Cd30l, Tnlg3a
Gene ID                         21949
Swissprot ID              P32972


Sample Type    Average % RecoveryRange %
Serum  9786-103
Plasma  9585-106
Cell culture media  9992-111


Sample TypeDilution% of Expected
Cell culture media1:299

Standard Curve: Mouse CD30L ELISA Kit

Mouse CD30L ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.


CD30 Ligand, also known as TNFSF8, is a type II membrane protein belonging to the TNF superfamily. CD30 Ligand is expressed on the surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse CD30L ELISA Kit is for Research Use Only.


1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected



There are no reviews yet.

Be the first to review “Mouse CD30L ELISA Kit”