Mouse CD30L ELISA Kit

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Mouse CD30L ELISA Kit
Mouse CD30L ELISA Kit Standard Curve

Mouse CD30L ELISA Kit

Catalog No : 3020312


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SKU: 3020312. Categories: , .

Mouse CD30L ELISA Kit Features

Product Name            Mouse CD30L ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   < 1 pg/mL
Assay Range                0.41-100 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names              Tnfsf8, CD153, CD30LG, Cd30l, Tnlg3a
Gene ID                         21949
Swissprot ID              P32972

Recovery

Sample Type    Average % RecoveryRange %
Serum  9786-103
Plasma  9585-106
Cell culture media  9992-111

Linearity

Sample TypeDilution% of Expected
Serum1:294
1:495
1:897
Plasma1:298
1:493
1:891
Cell culture media1:299
1:495
1:893

Standard Curve: Mouse CD30L ELISA Kit

Mouse CD30L ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CD30 Ligand, also known as TNFSF8, is a type II membrane protein belonging to the TNF superfamily. CD30 Ligand is expressed on the surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse CD30L ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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