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Mouse CCL6 ELISA Kit
Mouse CCL6 ELISA Kit Standard Curve

Mouse CCL6 ELISA Kit

Catalog No : 3020311


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SKU: 3020311. Category: .

Mouse CCL6 ELISA Kit Features

Product Name            Mouse CCL6 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   15 pg/mL
Assay Range                15.6-1000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Ccl6, MRP-1, Scya6, c10
Gene ID                         20305
Swissprot ID               P27784

Recovery

Sample Type    Average % RecoveryRange %
Serum  10295-115
Plasma   8980-96
Cell culture media   11095-121

Linearity

Sample TypeDilution% of Expected
Serum1:2104
1:4110
1:8105
Plasma1:2111
1:4107
1:8112
Cell culture media1:2101
1:499
1:8114

Standard Curve: Mouse CCL6 ELISA Kit

Mouse CCL6 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Mouse C10, a member of the CC subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homolog for mouse C10 has not been identified. The expression of C10 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid that can also be greatly induced in culture with GM-CSF.

However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11.The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse CCL6 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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