Mouse BSSP-4 ELISA Kit

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Mouse BSSP-4 ELISA Kit

Mouse BSSP-4 ELISA Kit

Catalog No : 3020341


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SKU: 3020341. Category: .

Mouse BSSP-4 ELISA Kit Features

Product Name            Mouse BSSP-4 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   50 pg/mL
Assay Range                62.5-4000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Prss22, 4733401N09Rik, BSSP-4, SP001LA
Gene ID                         70835
Swissprot ID               Q3U1Y4

Recovery

Sample Type    Average % RecoveryRange %
Serum9480-116
Plasma8575-97
Cell culture media8072-86

Linearity

Sample TypeDilution% of Expected
Serum1:2112
1:4104
1:8110
Plasma1:2113
1:4111
1:8106
Cell culture media1:2113
1:493
1:8109

Standard Curve: Mouse BSSP-4 ELISA Kit

Mouse BSSP-4 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Brain-specific serine protease 4 (BSSP-4), also known as serine protease 22 or tryptase epsilon, is an enzyme that in humans is encoded by the PRSS22 gene. Mouse Tryptase epsilon is preferentially expressed in epithelium-rich tissues such as the lung and eye, which is similar to its human counterpart. The mouse protein is synthesized with a signal peptide, a pro peptide and a mature chain corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse BSSP-4 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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