Mouse beta IG-H3 ELISA Kit

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Mouse beta IG-H3 ELISA Kit
Mouse betaIG-H3 ELISA Kit Standard Curve

Mouse beta IG-H3 ELISA Kit

Catalog No : 3020036


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SKU: 3020036. Category: .

Mouse beta IG-H3 ELISA Kit Features

Product Name            Mouse beta IG-H3 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   1 pg/mL
Assay Range                4.1-1000 pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Tgfbi, 68kDa, AI181842, AI747162, Beta-ig, big-h3
Gene ID                         21810
Swissprot ID               P82198

Recovery

Sample Type    Average % RecoveryRange %
Serum  133126-144
Plasma   129122-141
Cell culture media   134128-146

Linearity

Sample TypeDilution% of Expected
Serum1:2120
1:4122
1:8119
Plasma1:2124
1:4120
1:8126
Cell culture media1:2117
1:4125
1:8118

Standard Curve: Mouse beta IG-H3 ELISA Kit

Mouse beta IG-H3 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation.  TGFBI gene, locus 5q31, encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in manyextracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. Mutations of the gene cause several forms of corneal dystrophies.

Related Products

Human Beta IG-H3 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse beta IG-H3 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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