Mouse BAFF R ELISA Kit

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Mouse BAFF R ELISA Kit
Mouse BAFF R ELISA Kit Standard Curve

Mouse BAFF R ELISA Kit

Catalog No : 3020035


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SKU: 3020035. Categories: , .

Mouse BAFF R ELISA Kit Features

Product Name            Mouse BAFF R ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   15 pg/ml
Assay Range               28.7-7000 pg/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               2010006P15Rik, BAFF-R, Baffr, Bcmd, Bcmd-1, Bcmd1, Lvis22
Gene ID                        72049
Swissprot ID               Q9D8D0

Recovery

Sample Type    Average % RecoveryRange %
Serum125116-134
Plasma116107-125
Cell culture media9283-104

Linearity

Sample TypeDilution% of Expected
Serum1:2120
1:4128
1:8102
Plasma1:2121
1:4127
1:8104
Cell culture media1:2117
1:4115
1:8107

Standard Curve: Mouse BAFF R ELISA Kit

Mouse BAFF R ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. In humans it is encoded by the TNFRSF13C gene. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain.

It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

 Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse BAFF R ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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