Mouse ADAMTS1 ELISA Kit

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Mouse ADAMTS1 ELISA Kit
Mouse ADAMTS1 ELISA Kit

Mouse ADAMTS1 ELISA Kit

Catalog No : 3020363


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Mouse ADAMTS1 ELISA Kit Features

Product Name            Mouse ADAMTS1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   0.4 ng/mL
Assay Range                0.41-100 ng/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Adamts1, ADAM-TS1, ADAMTS, ADAMTS-1, C3-C5, METH-1, METH1
Gene ID                         11504
Swissprot ID               P97857

Recovery

Sample Type    Average % RecoveryRange %
Serum10495-114
Plasma10589-121
Cell culture media10795-117

Linearity

Sample TypeDilution% of Expected
Serum1:2101
1:4103
1:8104
Plasma1:2112
1:4115
1:8101
Cell culture media1:2122
1:4104
1:8101

Standard Curve: Mouse ADAMTS1 ELISA Kit

Mouse ADAMTS1 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multidomain structure.

Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in the degradation of cartilage leading to inflammation and arthritis. Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of a cofactor such as fibulin1.

ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs. It also plays an important role in atherosclerosis. It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF 2.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse ADAMTS1 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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