Product Name Human Pro-cathepsin B ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100 ul
Detection Method Colorimetric
Sensitivity 60 pg/ml
Assay Range 0.06-15 ng/ml
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names CTSB, APPS, CPSB
Gene ID 1508
Swissprot ID P07858
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 107 | 94-126 |
Plasma | 94 | 83-110 |
cell culture media | 75 | 70-82 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 92 |
1:4 | 81 | |
1:8 | 83 | |
Plasma | 1:2 | 97 |
1:4 | 91 | |
1:8 | 94 | |
cell culture media | 1:2 | 95 |
1:4 | 96 | |
1:8 | 98 |
Standard Curve: Human Pro-cathepsin B ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Cathepsin B is the first described member of the family of lysosomal cysteine proteases. Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase.
Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Cathepsin B may also have role in invasion and metastasis.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human Pro-cathepsin B ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
---|
Reviews
There are no reviews yet.