Human P-Cadherin ELISA Kit

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Human P-Cadherin ELISA Kit
Human P-Cadherin ELISA Kit Standard Curve

Human P-Cadherin ELISA Kit

Catalog No : 3010123


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SKU: 3010123. Category: .

Human P-Cadherin ELISA Kit Features

Product Name            Human P-Cadherin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   15 pg/mL
Assay Range                24.7-18000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CDHP, HJMD, PCAD
Gene ID                        1001
Swissprot ID               P22223

Recovery

Sample Type    Average % RecoveryRange %
Serum   11595-123
Plasma   11292-121
Cell culture media   120107-132

Linearity

Sample TypeDilution% of Expected
Serum1:2105
1:4121
1:8104
Plasma1:2112
1:4123
1:8114
Cell culture media1:2105
1:4119
1:8112

Standard Curve: Human P-Cadherin ELISA Kit

Human P-Cadherin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Cadherin is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, FAT family cadherins, and T-cadherin. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration.

Related Products

Custom mouse P-cadherin ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human P-Cadherin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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