Human MIP-1 beta ELISA Kit

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Human MIP-1 beta ELISA Kit
Human MIP-1 beta ELISA Kit Standard Curve

Human MIP-1 beta ELISA Kit

Catalog No : 3010109


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SKU: 3010109. Categories: , , .

Product Features

Product Name            Human MIP-1 beta ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   1pg/ml
Assay Range               4.1-1000pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ACT2, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, CCL4, LAG1, MIP1B, SCYA4
Gene ID                        6351
Swissprot ID              P13236

Recovery

Sample Type    Average % RecoveryRange %
Serum 9582-102
Plasma 9382-101
Cell culture media9889-110

Linearity

Sample TypeDilution% of Expected
Serum1:293
1:496
1:892
Plasma1:292
1:495
1:889
Cell culture media1:291
1:4102
1:890

Standard Curve: Human MIP-1 beta ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor. CCL4 has been shown to interact with CCL3.

Related Products

Human MIP-1 alpha ELISA Kit

Human MIP-1 delta ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human MIP-1 beta ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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