Human MIG ELISA Kit

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Human MIG ELISA Kit
Human MIG ELISA Kit Standard Curve

Human MIG ELISA Kit

Catalog No : 3010108


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SKU: 3010108. Categories: , , .

Human MIG ELISA Kit Features

Product Name            Human MIG ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   5 pg/ml
Assay Range               8.2-6000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CXCL9 CMK, MIG, SCYB9
Gene ID                        4283
Swissprot ID              Q07325

Recovery

Sample Type    Average % RecoveryRange %
Serum 9582-102
Plasma 9684-103
Cell culture media 10289-115

Linearity

Sample TypeDilution% of Expected
Serum1:294
1:496
1:892
Plasma1:296
1:498
1:889
Cell culture media1:298
1:4102
1:887

Standard Curve: Human MIG ELISA Kit

Human MIG ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

MIG, a member of the CXC subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. MIG is a chemoattractant for activated T cells and TIL, but not for neutrophils or monocytes. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. Neutrophil collagenase/matrix metalloproteinase 8 (MMP-8) degrades CXCL9 and cleaves CXCL10 at two positions. Gelatinase B/MMP-9 degrades CXCL10 and cleaves CXCL9 at three different sites in its extended carboxy-terminal region.

Related Products

Mouse MIG ELISA Kit

Custom porcine MIG ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human MIG ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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