Product Name Human Leptin ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum, Plasma, cell culture media , 100 ul
Detection Method Colorimetric
Sensitivity 8 pg/ml
Assay Range 8.2-2000 pg/ml
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names LEP, OB, OBS
Gene ID 3952
Swissprot ID P41159
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 94 | 82-103 |
Plasma | 92 | 84-101 |
Cell culture media | 95 | 87-105 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 92 |
1:4 | 95 | |
1:8 | 89 | |
Plasma | 1:2 | 93 |
1:4 | 91 | |
1:8 | 87 | |
Cell culture media | 1:2 | 91 |
1:4 | 93 | |
1:8 | 94 |
Standard Curve: Human Leptin ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human Leptin ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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