Product Name Human IL-32 alpha ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100 ul
Detection Method Colorimetric
Sensitivity 3 pg/ml
Assay Range 4.1-1000 pg/ml
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names IL32, IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF
Gene ID 9235
Swissprot ID P24001
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 113 | 98-124 |
Plasma | 111 | 101-120 |
cell culture media | 84 | 79-96 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 99 |
1:4 | 105 | |
1:8 | 103 | |
Plasma | 1:2 | 101 |
1:4 | 104 | |
1:8 | 107 | |
cell culture media | 1:2 | 106 |
1:4 | 125 | |
1:8 | 121 |
Standard Curve: Human IL-32 alpha ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human IL-32 alpha ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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