Product Name Human IL-1ra ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity 30pg/ml
Assay Range 41.1-30000pg/ml
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names IL1RN, IL1F3, IL1RA
Gene ID 3557
Swissprot ID P18510
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 97 | 87-106 |
Plasma | 99 | 89-108 |
Cell culture media | 96 | 87-105 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 98 |
1:4 | 97 | |
1:8 | 101 | |
Plasma | 1:2 | 102 |
1:4 | 95 | |
1:8 | 93 | |
Cell culture media | 1:2 | 101 |
1:4 | 94 | |
1:8 | 91 |
Standard Curve: Human IL-1ra ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
IL-1ra /IL-1F3 (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. It binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human IL-1ra ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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