Human IGF-II ELISA Kit

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Human IGF-II ELISA Kit
Human IGF-II ELISA Kit Standard Curve

Human IGF-II ELISA Kit

Catalog No : 3010359


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SKU: 3010359. Category: .

Human IGF-II ELISA Kit Features

Product Name           Human IGF-II ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   85 pg/ml
Assay Range               0.313-20ng/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               IGF2, C11orf43, GRDF, IGF-II, PP9974
Gene ID                        3481
Swissprot ID              P01344

Recovery

Sample Type    Average % RecoveryRange %
Serum  9087-93
Plasma  8980-95
Cell culture media  9182-94

Linearity

Sample TypeDilution% of Expected
Serum1:2101
1:4106
1:8110
Plasma1:2111
1:4108
1:898
Cell culture media1:2116
1:496
1:8102

Standard Curve: Human IGF-II ELISA Kit

Human IGF-II ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Insulin-like growth factor I and insulin-like growth factor II belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine an

d endocrine functions. IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development. Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. Circulating IGFs exist in complexes bound to IGF binding proteins.

Related Products

Human IGF-I ELISA Kit

Human IGF-II R ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human IGF-II ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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