Human ICAM-3 ELISA Kit

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Human ICAM-3 ELISA Kit
Human ICAM-3 ELISA Kit Standard Curve

Human ICAM-3 ELISA Kit

Catalog No : 3010208


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Human ICAM-3 ELISA Kit Features

Product Name            Human ICAM-3 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   35 pg/mL
Assay Range                65.5-16000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ICAM3, CD50, CDW50, ICAM-R
Gene ID                        3385
Swissprot ID              P32942

Recovery

Sample Type    Average % RecoveryRange %
Serum  119107-135
Plasma 112101-121
Cell culture media118110-127

Linearity

Sample TypeDilution% of Expected
Serum1:2107
1:4114
1:8115
Plasma1:2121
1:4120
1:8117
Cell culture media1:2118
1:4125
1:8119

Standard Curve: Human ICAM-3 ELISA Kit

Human ICAM-3 ELISA kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Intercellular adhesion molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alphad/beta2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response.[2] It functions not only as an adhesion molecule, but also as a potent signalling molecule.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human ICAM-3 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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