Human I-309 ELISA Kit

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Human I-309 ELISA Kit
Human I-309 ELISA Kit Standard Curve

Human I-309 ELISA Kit

Catalog No : 3010207


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SKU: 3010207. Categories: , .

Human I-309 ELISA Kit Features

Product Name            Human I-309 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   2 pg/mL
Assay Range                4.1-3000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CCL1, I-309, P500, SCYA1, SISe, TCA3
Gene ID                         6346
Swissprot ID               P22362

Recovery

Sample Type    Average % RecoveryRange %
Serum  10489-115
Plasma 9788-109
Cell culture media 8278-94

Linearity

Sample TypeDilution% of Expected
Serum1:2112
1:4121
1:8115
Plasma1:2114
1:4119
1:8117
Cell culture media1:2121
1:4123
1:8120

Standard Curve: Human I-309 ELISA Kit

Human I-309 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

I-309, a member of the CC subfamily of chemokines, was initially identified by subtractive hybridization as a transcript that was present in a gamma/delta T cell line, but not in EBV-transformed B cells. Human I-309 is assumed to be a homolog of the mouse TCA-3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.

Related Products

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human I-309 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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