Human HGF R ELISA Kit

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Human HGF R ELISA Kit
Human HGF R ELISA Kit Standard Curve

Human HGF R ELISA Kit

Catalog No : 3010205


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Human HGF R ELISA Kit Features

Product Name           Human HGF R ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   30 pg/mL
Assay Range                61.4-15000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               MET, AUTS9, DFNB97, HGFR, RCCP2, c-Met
Gene ID                         4233
Swissprot ID               P08581

Recovery

Sample Type    Average % RecoveryRange %
Serum  8879-103
Plasma 8172-94
Cell culture media 9081-111

Linearity

Sample TypeDilution% of Expected
Serum1:2120
1:4104
1:8108
Plasma1:2117
1:4102
1:8109
Cell culture media1:2118
1:4113
1:8110

Standard Curve: Human HGF R ELISA Kit

Human HGF R ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

HGF receptor, a product of the proto-oncogene c-met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. c-MET is synthesized as a single-chain precursor (pr170) which undergoes post-translational glycosylation and proteolytic cleavage to give rise to the heterodimeric mature form. MET activation by its ligand HGF induces MET kinase catalytic activity, which triggers transphosphorylation of the tyrosines Tyr 1234 and Tyr 1235. These two tyrosines engage various signal transducers, thus initiating a whole spectrum of biological activities driven by MET, collectively known as the invasive growth program. The transducers interact with the intracellular multisubstrate docking site of MET either directly, such as GRB2, SHC, SRC, and the p85 regulatory subunit of phosphatidylinositol-3 kinase (PI3K), or indirectly through the scaffolding protein Gab1.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human HGF R ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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