Human HCC-1 EasyTest™ ELISA Kit

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Human HCC-1 ELISA Kit
Human HCC-1 ELISA Kit

Human HCC-1 EasyTest™ ELISA Kit

Catalog No : 1010165


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SKU: 1010165. Category: .

Product Features

Product Name           Human HCC-1 EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   15 pg/mL
Assay Range               31.3-2000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CCL14, CKB1, HCC-1, HCC-1/HCC-3, HCC-3, MCIF, NCC-2, NCC2, SCYA14, SCYL2, SY14
Gene ID                         6358
Swissprot ID               Q14498

Recovery

Sample Type    Average % RecoveryRange %
Serum    10492-123
Plasma    10195-117

Linearity

Sample TypeDilution% of Expected
Serum1:294
1:481
1:892
Plasma1:2102
1:484
1:897

Standard Curve: Human HCC-1 EasyTest™ ELISA Kit

Human HCC-1 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human TNF-alpha mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

CCL14, is a member of the chemokine superfamily of ligands. In-depth genome analysis has determined that there is no mouse ortholog for human CCL14. Two alternatively spliced isoforms of CCL14, CCL14a/HCC-1 and CCL14b/HHC-3, have been identified. CCL14a/HCC-1 is cleaved into a 74 aa secreted bioactive peptide by Plasmin or u-Plasminogen Activator/Urokinase. CCL14a/HCC-1 binds and activates CCR1, CCR3, and CCR5; it also binds the non-signaling chemokine decoy receptors DARC and D6. CCL14a/HCC-1 is widely expressed in normal tissues including spleen, liver, skeletal and heart muscle, gut, and bone marrow, and it circulates at high concentrations in the plasma.

CCL14a/HCC-1 induces the chemotaxis of T lymphocytes, monocytes, and eosinophils. It also exhibits anti-HIV activities by causing CCR5, a key mediator of HIV entry, internalization and intracellular sequestration. CCL14 is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human HCC-1 EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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