Human Growth hormone ELISA Kit

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Human Growth hormone ELISA Kit
Human Growth hormone ELISA Kit Standard Curve

Human Growth hormone ELISA Kit

Catalog No : 3010072


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SKU: 3010072. Categories: , .

Human Growth hormone ELISA Kit Features

Product Name           Human Growth hormone ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   <1 pg/ml
Assay Range                2.5-600 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               GH1; GH; GH-N; GHB5; GHN; IGHD1B; hGH-N
Gene ID                        2688
Swissprot ID              P01241

Recovery

Sample Type    Average % RecoveryRange %
Serum 9480-102
Plasma 9585-106
Cell culture media 9282-101

Recovery

Sample TypeDilution% of Expected
Serum1:289
1:4101
1:8101
Plasma1:292
1:493
1:898
Cell culture media1:290
1:497
1:894

Standard Curve: Human Growth hormone ELISA Kit

Human Growth hormone ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Growth hormone (GH), also known as somatotropin (or as human growth hormone [hGH or HGH] in its human form), is apeptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is thus important inhuman development. It is a type of mitogen which is specific only to certain kinds of cells. Growth hormone is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland. GH is a stress hormone that raises the concentration of glucose and free fatty acids. It also stimulates production of IGF-1.

Secretion of growth hormone (GH) in the pituitary is regulated by the neurosecretory nuclei of the hypothalamus. These cells release the peptides Growth hormone-releasing hormone (GHRH or somatocrinin) and Growth hormone-inhibiting hormone (GHIH or somatostatin) into the hypophyseal portal venous blood surrounding the pituitary. GH release in the pituitary is primarily determined by the balance of these two peptides, which in turn is affected by many physiological stimulators (e.g., exercise, nutrition, sleep) and inhibitors (e.g., free fatty acids) of GH secretion.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Growth hormone ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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