Human GRO-alpha ELISA Kit

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Human GRO-alpha ELISA Kit
Human GRO-alpha ELISA kit

Human GRO-alpha ELISA Kit

Catalog No : 3010100


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SKU: 3010100. Categories: , .

Human GRO-alpha ELISA Kit Features

Product Name           Human GRO-alpha ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   1 pg/mL
Assay Range                1.37-1000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CXCL1, FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1
Gene ID                         2919
Swissprot ID               P09341

Recovery

Sample Type    Average % RecoveryRange %
Serum   127110-134
Plasma  11390-128
cell culture media   11099-121

Linearity

Sample TypeDilution% of Expected
Serum1:2102
1:4104
1:8101
Plasma1:2122
1:4112
1:897
cell culture media1:292
1:489
1:893

 Standard Curve: Human GRO-alpha ELISA Kit

Human GRO-alpha ELISA kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

The chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α).

CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. It plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, arteriogenesis, inflammation, wound healing, and tumorigenesis. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human GRO-alpha ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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