Human Galectin-9 ELISA Kit

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Human Galectin-9 ELIS kit
Human Galectin-9 ELISA Kit

Human Galectin-9 ELISA Kit

Catalog No : 3010462


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Human Galectin-9 ELISA Kit Features

Product Name           Human Galectin-9 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   35 pg/mL
Assay Range                36.9-9000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               LGALS9; HUATA, LGALS9
Gene ID                         3965
Swissprot ID               O00182

Recovery

Sample Type    Average % RecoveryRange %
Serum   8577-93
Plasma  8475-91
cell culture media   112101-127

Linearity

Sample TypeDilution% of Expected
Serum1:2101
1:496
1:890
Plasma1:2114
1:4104
1:8112
cell culture media1:2108
1:4102
1:8111

 Standard Curve: Human Galectin-9 ELISA Kit

Human Galectin-9 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta -galactosidase binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Galectin-9 is strongly overexpressed in Hodgkin’s disease tissue and it might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its consistently associated immunodeficiency.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Galectin-9 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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