Human Furin ELISA Kit

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Human Furin ELISA Kit
Human Furin ELISA Kit Standard Curve

Human Furin ELISA Kit

Catalog No : 3010068


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Human Furin ELISA Kit Features

Product Name           Human Furin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   8 pg/ml
Assay Range               13.7-10000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               FURIN; FUR; PACE; PCSK3; SPC1
Gene ID                        5045
Swissprot ID              P09958

Recovery

Sample Type    Average % RecoveryRange %
Serum 8981-96
Plasma 7976-92
Cell culture media 8577-93

Linearity

Sample TypeDilution% of Expected
Serum1:299
1:479
1:896
Plasma1:2102
1:481
1:898
Cell culture media1:2116
1:4103
1:895

Standard Curve: Human Furin ELISA Kit

Human Furin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Furin is also known as PACE (Paired basic Amino acid Cleaving Enzyme). This family of serine proteases consists of furin and proprotein convertases encoded by PCSK genes. They are important in a variety of processes. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. Mutations in the PC1/PCSK1 gene are associated with obesity and impaired prohormone processing.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Furin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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