Human FSH ELISA Kit

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Human FSH ELISA Kit
Human FSH ELISA Kit

Human FSH ELISA Kit

Catalog No : 3010460


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SKU: 3010460. Category: .

Human FSH ELISA Kit Features

Product Name           Human FSH ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   6 pg/mL
Assay Range                8.2-2000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CGA;  FSHA; FSHB
Gene ID                         1081/2488
Swissprot ID               P01225

Recovery

Sample Type    Average % RecoveryRange %
Serum   9282-103
Plasma  9079-94
cell culture media   9181-101

Linearity

Sample TypeDilution% of Expected
Serum1:296
1:4107
1:8111
Plasma1:2101
1:4117
1:8112
cell culture media1:2109
1:4121
1:8117

 Standard Curve: Human FSH ELISA Kit

Human FSH ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Follicle-Stimulating Hormone (FSH) is a 33 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit is also a component of Luteinizing Hormone (LH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with the FSH Receptor.

FSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, FSH secretion can also be stimulated by activins and inhibited by inhibins. FSH works in concert with LH to regulate female reproduction; It stimulates follicular growth and LH induces ovulation. FSH can also regulate estrogen formation by inducing the aromatase enzyme that converts androgen to estradiol.

In males, FSH is critical for sperm production. Mutations in the human FSH and FSHR genes are associated with reduced reproductive functioning in females. Additionally, increased production of autoantibodies directed against FSH beta has been suggested to contribute to certain types of female infertility. Assisted reproductive technology commonly uses recombinant FSH to induce follicle growth.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human FSH ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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