Human FGF-9 ELISA Kit

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Human FGF-9 ELISA Kit
Human FGF-9 ELISA Kit Standard Curve

Human FGF-9 ELISA Kit

Catalog No : 3010198


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Human FGF-9 ELISA Kit Features

Product Name           Human FGF-9 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   100 pg/mL
Assay Range                143.4-35000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               FGF9, FGF-9, GAF, HBFG-9, HBGF-9, SYNS3
Gene ID                         2254
Swissprot ID              P31371

Recovery

Sample Type    Average % RecoveryRange %
Serum  9886-110
Plasma 9585-105
Cell culture media 10192-113

Linearity

Sample TypeDilution% of Expected
Serum1:295
1:497
1:892
Plasma1:296
1:494
1:893
Cell culture media1:298
1:495
1:897

Standard Curve: Human FGF-9 ELISA Kit

Human FGF-9 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

FGF-9 (Fibroblast Growth Factor-9), also known as HBGF-9 and GAF, is a heparin-binding protein that regulates cell proliferation, differentiation, and function through interactions with FGF R3 (IIIb) and possibly FGF R3 (IIIc). The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human FGF-9 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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