Human FGF-4 ELISA Kit

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Human FGF-4 ELISA Kit
Human FGF-4 ELISA Kit Standard Curve

Human FGF-4 ELISA Kit

Catalog No : 3010064


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Human FGF-4 ELISA Kit Features

Product Name           Human FGF-4 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   15 pg/ml
Assay Range               24.7-18000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF
Gene ID                        2249
Swissprot ID              P08620

Recovery

Sample Type    Average % RecoveryRange %
Serum 9788-112
Plasma 9482-107
Cell culture media 9180-103

Linearity

Sample TypeDilution% of Expected
Serum1:294
1:493
1:891
Plasma1:297
1:494
1:898
Cell culture media1:296
1:495
1:8101

Standard Curve: Human FGF-4 ELISA Kit

Human FGF-4 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

FGF-4 (Fibroblast Growth Factor-4), also known as FGF-K and K-FGF, is a secreted angiogenic factor that is mitogenic for fibroblasts and endothelial cells. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. During development, it is required for maintenance of the trophoectoderm and primitive endoderm, and it cooperates with FGF-8 in the apical ectodermal ridge. FGF-4 promotes embryonic stem cell proliferation and may also aid stem cell differentiation.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human FGF-4 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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