Human FGF-19 ELISA Kit

Human FGF-19 ELISA Kit Features

Product Name           Human FGF-19 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   20 pg/mL
Assay Range                32.8-8000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               FGF19
Gene ID                          9965
Swissprot ID               O95750

Recovery

Linearity

Standard Curve: Human FGF-19 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

FGF-19 (Fibroblast Growth Factor-19) is a secreted heparin-binding protein in the endocrine subfamily of FGFs that also includes FGF-21 and FGF-23. It is produced by epithelial cells of the ileal intestine in response to bile acids and signals through a receptor complex of FGF R4 and Klotho-beta in the liver. FGF-19 suppresses bile acid synthesis and enhances hepatic protein and glycogen synthesis. Dysregulalted FGF-19 activity is associated with the development of hypercholesterolemia, gall stones, bile acid induced diarrhoea (BAD), hepatocellular cancer, colon cancer, and metabolic syndrome. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family.

FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development.

Related Products

Human FGF-4 ELISA Kit

Human FGF-5 ELISA Kit

Human FGF-6 ELISA Kit

Human FGF-7 ELISA Kit

Human FGF-9 ELISA Kit

Human FGF-21 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human FGF-19 ELISA Kit is for Research Use Only.

• Manual
• Assay procedure
• MSDS