Catalog No : 3010194
Product Name Human FGF-19 ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity 20 pg/mL
Assay Range 32.8-8000 pg/mL
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names FGF19
Gene ID 9965
Swissprot ID O95750
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 104 | 94-116 |
Plasma | 106 | 89-123 |
Cell culture media | 96 | 84-107 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 115 |
1:4 | 99 | |
1:8 | 104 | |
Plasma | 1:2 | 110 |
1:4 | 115 | |
1:8 | 103 | |
Cell culture media | 1:2 | 120 |
1:4 | 112 | |
1:8 | 110 |
Standard Curve: Human FGF-19 ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
FGF-19 (Fibroblast Growth Factor-19) is a secreted heparin-binding protein in the endocrine subfamily of FGFs that also includes FGF-21 and FGF-23. It is produced by epithelial cells of the ileal intestine in response to bile acids and signals through a receptor complex of FGF R4 and Klotho-beta in the liver. FGF-19 suppresses bile acid synthesis and enhances hepatic protein and glycogen synthesis. Dysregulalted FGF-19 activity is associated with the development of hypercholesterolemia, gall stones, bile acid induced diarrhoea (BAD), hepatocellular cancer, colon cancer, and metabolic syndrome. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family.
FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human FGF-19 ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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Species Detected | Human |
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