Human Fc gamma RIIB/C ELISA Kit

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Human Fc gamma RIIB/C ELISA Kit
Human Fc gamma RIIB/C ELISA Kit Standard Curve

Human Fc gamma RIIB/C ELISA Kit

Catalog No : 3010193


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Human Fc gamma RIIB/C ELISA Kit Features

Product Name            Human Fc gamma RIIB/C ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   60 pg/mL
Assay Range                78.1-5000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               FCGR2B, CD32, CD32B, FCG2, FCGR2, IGFR2
Gene ID                         2213
Swissprot ID               P31994

Recovery

Sample Type    Average % RecoveryRange %
Serum  9991-108
Plasma  110101-121
Cell culture media  8981-92

Linearity

Sample TypeDilution% of Expected
Serum1:287
1:481
1:889
Plasma1:296
1:482
1:886
Cell culture media1:294
1:484
1:892

Standard Curve: Human Fc gamma RIIB/C ELISA Kit

Human Fc-gamma RII B/C ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. In humans each group of receptors is encoded by 2 – 3 closely related genes designated A, B, and C. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Different Fc receptors can function as activators, as inhibitors, or as decoy receptors. The inhibitory CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human Fc gamma RIIB/C ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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