Human ErbB3 ELISA Kit

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Human ErbB3 ELISA Kit
Human ErbB3 ELISA Kit Standard Curve

Human ErbB3 ELISA Kit

Catalog No : 3010189


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Human ErbB3 ELISA Kit Features

Product Name            Human ErbB3 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   2 pg/mL
Assay Range                3.4-2500 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ERBB3, ErbB-3, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3
Gene ID                         2065
Swissprot ID               P21860

Recovery

Sample Type    Average % RecoveryRange %
Serum  9578-106
Plasma  9080-104
Cell culture media  9784-114

Linearity

Sample TypeDilution% of Expected
Serum1:293
1:495
1:892
Plasma1:296
1:497
1:8101
Cell culture media1:2101
1:489
1:894

Standard Curve: Human ErbB3 ELISA Kit

Human ErbB3 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

ErbB3, also called Her3 in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB3 binds neuregulins NRG1 and NRG2, but lacks a working kinase domain; heterodimerization with another ErbB receptor is required for signaling. ErbB3 is important in development of Schwann cells, neural crest cells, heart valves and the sympathetic nervous system.

ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosphorylation by its binding partner to be active. ErbB3 was found to be kinase impaired, having only 1/1000 the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins.Therefore, ErbB3 must act as an allosteric activator. ErbB3 binding causes the allosteric activation of p110, the lipid kinase subunit of PI3K, a function not found in either EGFR or ErbB2.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human ErbB3 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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